maldi ms imaging
matrix-assisted laser desorption/ionization mass spectrometric imaging requires a matrix to applied to the sample before imaging. with the iMatrixSpray this is done with a few mouse clicks:
solution A: 10 mL, 10 mg/ml CHCA in 50:50 ACN/0.1% TFA
hight: 60 mm
line distance: 1 mm
speed: 180 mm/s
density: 3 μL/cm²
cycles: 10
delay: 0 s
the systems starts with a rinsing cycle, delivers the spray and cleans the system afterwards. all fully automatic, no user intervention required.
quantification
successful quantification with surface analysis techniques requires a careful experiment design, involving the homogeneous and reproducible application of an internal standard. the iMatrixSpray can do this with ease, requiring just few user inputs. in this example, we use solution B to hold our standard.
solution B: 1 mL, 50 μM standard 50:50 ACN/water
hight: 60 mm
line distance: 1 mm
speed: 180 mm/s
density: 1 μL/cm²
cycles: 1
delay: 0 s
no limits
you want to execute your own commands and scripts on the iMatrixSpray? check the support section on this website on the available iMatrixSpray command and sample scripts to get you started with your custom project.